Segara Anakan Estuarine known as one of the potential estuarine and become important habitat in providing space for fauna aquatic life, especially fish . The purpose of this study was to determine the appropriate location for the development of fish sanctuary in Segara Anakan Estuarine. Location of the study covers the whole region Segara Anakan estuarine with 13 stations in estuarine waters body and 8 Coastal Village. Data collected by using survey method (stratified sampling method). Assessment sutability for fish sanctuary development using ecological integrity and connectivity of social ecology approach, includes 22 parameters, while the analysis of the data using spatial analysis approach. The results of research obtained are very appropriate class of 1204.29 ha (scattered in Klaces, Kutawaru, Kembang Kuning river and Tritih), corresponding conditional class 1579.71 ha (scattered Ujung Alang, Panikel, partly Kutawaru waters and tritih) , and the class is not appropriate for 888.49 ha (scattered in Donan, Talun, and Ujung Gagak).
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